NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]
cat.: HA751003
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NCOA4 aa 1-400 / 614. |
| Positive control: | HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q13772 Human |
| Alternative names: | 70 kDa androgen receptor activator 70 kDa androgen receptor coactivator 70 kDa AR activator 70 kDa AR-activator Androgen receptor coactivator 70 kD Androgen receptor coactivator 70 kDa protein Androgen receptor-associated protein of 70 kDa ARA 70 ARA70 DKFZp762E1112 ELE 1 ELE1 ELE1/ret TK NCOA 4 NCoA-4 NCOA4 NCOA4_HUMAN Nuclear receptor coactivator 4 Papillary thyroid carcinoma 3 PTC 3 PTC3 RET activating gene ELE1 Ret activating protein ELE1 Ret-activating protein ELE1 RFG |
Images
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Fig1:
Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA751003) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A-172 cell lysate Lane 3: A549 cell lysate Lane 4: A431 cell lysate Lane 5: HL-60 cell lysate Lane 6: NIH:OVCAR-3 cell lysate Lane 7: A375 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751003) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NCOA4 on different lysates with Rabbit anti-NCOA4 antibody (HA751003) at 1/5,000 dilution. Lane 1: KYSE150-si NT cell lysate Lane 2: KYSE150-si NCOA4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751003) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.