GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91]
cat.: HA751027
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IP, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH05-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse brain tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P IP IHC-Fr |
1:1,000 1:1,000 1-2μg/sample 1:500 |
| Uniprot #: | SwissProt: P48051 Human | P48542 Mouse | P48550 Rat |
| Alternative names: | inwardly rectifying subfamily J member 6 BIR1 G protein activated inward rectifier potassium channel 2 G protein-activated inward rectifier potassium channel 2 GIRK-2 GIRK2 hiGIRK2 Inward rectifier K(+) channel Kir3.2 Inward rectifier potassium channel KIR3.2 KATP-2 KATP2 KCNJ6 KCNJ6_HUMAN Kcnj7 KIR3.2 KPLBS Potassium channel Potassium channel inwardly rectifying subfamily J member 6 Potassium inwardly rectifying channel subfamily J member 6 Potassium voltage gated channel subfamily J member 6 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig2:
Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Rat hippocampus tissue lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 48 kDa Observed band size: 35/48 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751027 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751027 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA751027 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751027 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds; ECL: K1801 |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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