AIDA Recombinant Rabbit Monoclonal Antibody [PSH05-81]
cat.: HA751029
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AIDA aa 1-306. |
| Positive control: | HeLa cell lysate, U-87 MG cell lysate, Jurkat cell lysate, 293T cell lysate, MEF cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96BJ3 Human | Q8C4Q6 Mouse Entrez Gene: 682999 Rat |
| Alternative names: | 2610208M17Rik AIDA AIDA_HUMAN Axin interaction partner and dorsalization antagonist Axin interactor Axin interactor dorsalization associated protein Axin interactor, dorsalization associated C1orf80 Chromosome 1 open reading frame 80 dorsalization-associated protein RP11-378J18.7 UPF0491 protein C1orf80 |
Images
|
Fig1:
Western blot analysis of AIDA on different lysates with Rabbit anti-AIDA antibody (HA751029) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-87 MG cell lysate Lane 3: Jurkat cell lysate Lane 4: 293T cell lysate Lane 5: MEF cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: C2C12 cell lysate Lane 9: Neuro-2a cell lysate Lane 10: PC-12 cell lysate Lane 11: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time (left): 3 minutes; ECL: K1801; Exposure time (right): 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751029) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.