Caspase-8 (p18) Recombinant Rabbit Monoclonal Antibody [PSH05-83]
cat.: HA751031
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH05-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein. |
| Positive control: | RAW264.7 cell lysate, RAW264.7 treated with 1μM Etoposide for 18 hours cell lysate, CTLL-2 cell lysate, RAW264.7 cells treated with 1μM Etoposide for 18 hours. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: O89110 Mouse |
| Alternative names: | ALPS2B Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein Apoptosis related cysteine peptidase Apoptotic cysteine protease Apoptotic protease Mch-5 Apoptotic protease Mch5 CAP 4 CAP4 CASP-8 CASP8 CASP8_HUMAN Caspase 8 Caspase 8 apoptosis related cysteine peptidase Caspase IIX Caspase-8 subunit p10 caspase8 CED 3 FADD Homologous ICE/CED3 Like Protease FADD Like ICE FADD-homologous ICE/CED-3-like protease FADD-like ICE FLICE FLJ17672 ICE-like apoptotic protease 5 MACH alpha 1/2/3 protein MACH MACH beta 1/2/3/4 protein MACH5 MCH 5 MCH5 MGC78473 MORT1 associated ced 3 homolog MORT1 associated CED3 homolog MORT1-associated CED-3 homolog OTTHUMP00000163717 OTTHUMP00000163720 OTTHUMP00000163724 OTTHUMP00000163725 OTTHUMP00000165062 OTTHUMP00000165063 OTTHUMP00000165064 OTTHUMP00000206552 OTTHUMP00000206582 |
Images
|
Fig1:
Western blot analysis of Caspase-8 (p18) on different lysates with Rabbit anti-Caspase-8 (p18) antibody (HA751031) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1μM Etoposide for 18 hours cell lysate Lane 3: CTLL-2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 43/18 kDa Observed band size: 43/18 kDa Exposure time: 1 minute 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751031) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of RAW264.7 cells treated with 1μM Etoposide for 18 hours labeling Caspase-8 (p18) with Rabbit anti-Caspase-8 (p18) antibody (HA751031) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-8 (p18) antibody (HA751031) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.