PHACTR3 Recombinant Rabbit Monoclonal Antibody [PSH06-09]
cat.: HA751047
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH06-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PHACTR3 aa 401-450. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2. |
| Subcellular location: | Nucleus matrix. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: Q96KR7 Human |
| Alternative names: | Phosphatase and actin regulator 3 Scaffold-associated PP1-inhibiting protein (Scapinin) PHACTR3 C20orf101 SCAPIN1 |
Images
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Fig1:
Western blot analysis of PHACTR3 on different lysates with Rabbit anti-PHACTR3 antibody (HA751047) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751047) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling PHACTR3 with Rabbit anti-PHACTR3 antibody (HA751047) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PHACTR3 antibody (HA751047) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.