GAD65 Recombinant Rabbit Monoclonal Antibody [PSH06-80]
cat.: HA751102
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH06-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr |
1:2,000-1:5,000 1:1,000 1:500 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q05329 Human | P48320 Mouse | Q05683 Rat |
| Alternative names: | 65 kDa glutamic acid decarboxylase DCE 2 DCE2 DCE2_HUMAN GAD 2 GAD 65 GAD-2 GAD-65 Gad2 Glutamate decarboxylase 2 (pancreas) Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) Glutamate decarboxylase 2 Glutamate Decarboxylase 65 Glutamate decarboxylase 65 kDa isoform Glutamic Acid Decarboxylase 2 Glutamic Acid Decarboxylase 65 MGC161605 MGC161607 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 (GAD65, HA751102, Rabbit, green); 1/1,000 (GFAP, HA610317, Rat, red) Antigen retrieval: Not required |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GAD65 antibody (HA751102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GAD65 antibody (HA751102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GAD65 antibody (HA751102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Western blot analysis of GAD65 on different lysates with Rabbit anti-GAD65 antibody (HA751102) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat cerebellum tissue lysate (20 µg/Lane) Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751102) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig9:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.