SLC16A3 / MCT 4 Recombinant Rabbit Monoclonal Antibody [PSH06-91]
cat.: HA751115
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SLC16A3 aa 385-465. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, HCT 116 cell lysate, NCI-H441 cell lysate, COS-1 cell lysate, HeLa, human placenta tissue, human rectum tissue, human skeletal muscle tissue. |
| Subcellular location: | Cell membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:20,000 1:100 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O15427 Human |
| Alternative names: | MCT 4 MCT3 MCT4 Monocarboxylate transporter 3 Monocarboxylate transporter 4 MOT4_HUMAN SLC16A3 Solute carrier family 16 member 3 |
Images
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Fig1:
Western blot analysis of SLC16A3 / MCT 4 on different lysates with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate (negative) Lane 4: HCT 116 cell lysate Lane 5: NCI-H441 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 40 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751115) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling SLC16A3 / MCT 4 with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751115) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human rectum tissue with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751115) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-SLC16A3 / MCT 4 antibody (HA751115) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751115) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HeLa cells labeling SLC16A3 / MCT 4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751115, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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