ZO1 Recombinant Rabbit Monoclonal Antibody [PSH07-09]
cat.: HA751131
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey, Cynomolgus monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH07-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 195 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ZO1 aa 1,401-1,748. |
| Positive control: | A431 cell lysate, 293T cell lysate, HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, MCF7. |
| Subcellular location: | Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q07157 Human |
| Alternative names: | Tight junction protein 1 Tight junction protein ZO-1 Tight junction protein ZO1 TJP1 zo-1 Zo1 ZO1_HUMAN Zona occludens 1 Zona occludens 1 protein Zona occludens protein 1 Zonula occludens 1 protein Zonula occludens protein 1 |
Images
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Fig1:
Western blot analysis of ZO1 on different lysates with Rabbit anti-ZO1 antibody (HA751131) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: U-2 OS cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: C2C12 cell lysate (20 µg/Lane) Lane 8: COS-1 cell lysate (20 µg/Lane) Lane 9: Mouse testis tissue lysate (40 µg/Lane) Lane 10: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 195 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of MCF7 cells labeling ZO1 with Rabbit anti-ZO1 antibody (HA751131) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZO1 antibody (HA751131) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.