ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH07-35]
cat.: HA751156
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ANGPTL4 aa 107-406. |
| Positive control: | A549 cell lysate, SW480 cell lysate, THP-1 cell lysate, HUVEC cell lysate, COS-1 cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9BY76 Human |
| Alternative names: | Angiopoietin like 4 Angiopoietin related protein 4 Angiopoietin-like protein 4 Angiopoietin-related protein 4 ANGL4_HUMAN ANGPT L2 ANGPT L4 ANGPTL2 Angptl4 ARP4 Fasting induced adipose factor FIAF HARP Hepatic angiopoietin related protein Hepatic fibrinogen/angiopoietin related protein Hepatic fibrinogen/angiopoietin-related protein HFARP NL2 Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein PGAR pp1158 PPARG angiopoietin related protein PSEC0166 TGQTL UNQ171 Weakly similar to angiopoietin 1 [H.sapiens] |
Images
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Fig1:
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA751156) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SW480 cell lysate Lane 3: THP-1 cell lysate Lane 4: HUVEC cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA751156) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ANGPTL4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751156) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.