CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-02]
cat.: HA751202
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-P, FC, IF-Cell, Depletion |
| Clonality: | Monoclonal |
| Clone number: | PSH08-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD41 aa 451-989. |
| Positive control: | Mouse placenta tissue, mouse spleen tissue, mouse platelets. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P FC IF-Cell |
1:200-1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9QUM0 Mouse |
| Alternative names: | antigen CD41 BDPLT16 BDPLT2 CD41 CD41B form 2 GP2B GPalpha IIb GPIIb GT GTA HPA3 Integrin alpha 2b Integrin alpha IIb Integrin alpha-IIb light chain Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) ITA2B_HUMAN Itga2b ITGAB platelet fibrinogen receptor, alpha subunit platelet glycoprotein IIb of IIb/IIIa complex Platelet membrane glycoprotein IIb platelet specific antigen BAK PPP1R93 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (HA751202) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (HA751202) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751202) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of mouse platelets labeling CD41. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751202, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4: Immunocytochemistry analysis of mouse platelets labeling CD41 with Rabbit anti-CD41 antibody (HA751202, cyan) at 1/1,000 dilution. |
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Fig5: Validation of anti-CD41 antibody depletion function in vivo. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.