Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]
cat.: HA751215
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH08-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OXT aa 1-125. |
| Positive control: | Mouse paraventricular nucleus tissue, rat supraoptic nucleus tissue, mouse pituitary tissue, rat pituitary tissue, Mouse pituitary tissue lysate, Rat pituitary tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:1,000 1:2,000-1:5,000 1:500-1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P01178 Human | P35454 Mouse Entrez Gene: 25504 Rat |
| Alternative names: | MGC126890 MGC126892 Neurophysin 1 Neurophysin I Ocytocin OT OT NPI OXT Oxytocin Oxytocin Neurophysin preproprotein Oxytocin prepro (neurophysin I) Oxytocin prepropeptide Oxytocin-neurophysin 1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Paraventricular nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Supraoptic nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IF-tissue Species: Mouse Site: Pituitary Sample: Paraffin-embedded section Antibody concentration: 1/2,000 |
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Fig4:
Application: IF-tissue Species: Rat Site: Pituitary Sample: Paraffin-embedded section Antibody concentration: 1/2,000 |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse paraventricular nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig9:
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/1,000 dilution. Lane 1: Mouse pituitary tissue lysate Lane 2: Rat pituitary tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 13 kDa Observed band size: 14 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751215) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.