HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52]
cat.: HA751227
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH08-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HMGCS1 aa 421-520. |
| Positive control: | HepG2 cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, U-2 OS cell lysate, human liver tissue, mouse liver tissue, rat liver tissue, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:2,000 1:200-1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q01581 Human | Q8JZK9 Mouse | P17425 Rat |
| Alternative names: | 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) 3-hydroxy-3-methylglutaryl coenzyme A synthase cytoplasmic EC 2.3.3.10 HMCS1_HUMAN HMG CoA synthase HMG-CoA synthase HMGCS HMGCS1 Hydroxymethylglutaryl CoA synthase, cytoplasmic Hydroxymethylglutaryl-CoA synthase MGC90332 |
Images
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Fig1:
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA751227) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: U-2 OS cell lysate (20 µg/Lane) Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA751227 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751227 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: HA751227 IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of HA751227 in HT-29 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 37 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.