Ghrelin Recombinant Rabbit Monoclonal Antibody [PSH08-77]
cat.: HA751242
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH08-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Ghrelin aa 1-117. |
| Positive control: | Human stomach tissue, mouse stomach tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IHC-Fr IF-Tissue |
1:25,000-1:50,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: Q9UBU3 Human | Q9EQX0 Mouse |
| Alternative names: | Appetite regulating hormone Ghrelin 27 Ghrelin 28 Ghrelin and obestatin prepropeptide Ghrelin Ghrelin/obestatin prepropeptide ghrl GHRL_HUMAN Growth hormone releasing peptide Growth hormone secretagogue Growth hormone-releasing peptide M46 protein Motilin related peptide Motilin-related peptide MTLRP Obestatin Protein M46 |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse stomach tissue with Rabbit anti-Ghrelin antibody (HA751242) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751242, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of frozen mouse liver tissue (negative) with Rabbit anti-Ghrelin antibody (HA751242) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751242, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Ghrelin antibody (HA751242) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751242) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Ghrelin antibody (HA751242) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751242) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ghrelin antibody (HA751242) at 1/25,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751242) at 1/25,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Application: IF-Tissue Species: Mouse Site: stomach Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.