Phosphoserine aminotransferase Recombinant Rabbit Monoclonal Antibody [PSH08-99]
cat.: HA751260
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH08-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSAT1 aa 1-370. |
| Positive control: | NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, human colon cancer tissue, human kidney tissue, rat kidney tissue, HeLa. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:2,000 1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9Y617 Human | Q99K85 Mouse Entrez Gene: 293820 Rat |
| Alternative names: | EC 2.6.1.52 Endometrial progesterone induced protein EPIP MGC1460 NLS2 Phosphohydroxythreonine aminotransferase phosphoserine aminotransferase 1 Phosphoserine aminotransferase PSA PSAT Psat1 PSATD SERC_HUMAN |
Images
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Fig1:
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: mouse brain tissue lysate (40 µg/Lane) Lane 3: rat brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751260) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751260) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HeLa cells labeling Phosphoserine aminotransferase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751260, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7:
Phosphoserine aminotransferase was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751260 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751260 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751260 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751260 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
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