SLP76 Recombinant Rabbit Monoclonal Antibody [PSH09-22]
cat.: HA751276
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SLP76 aa 1- 533/533 |
| Positive control: | THP-1 cell lysate, HL-60 cell lysate, THP-1, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell IP |
1:2,000 1:5,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q13094 Human |
| Alternative names: | CG8697 LCP 2 LCP2 LCP2_HUMAN Lymphocyte cytosolic protein 2 SH2 domain containing leukocyte protein 76 KD SH2 domain containing leukocyte protein of 76kD SH2 domain containing leukocyte protein of 76kDa SH2 domain-containing leukocyte protein of 76 kDa SLP 76 SLP 76 tyrosine phosphoprotein SLP-76 SLP-76 tyrosine phosphoprotein SLP76 SLP76 tyrosine phosphoprotein |
Images
|
Fig1:
Western blot analysis of SLP76 on different lysates with Rabbit anti-SLP76 antibody (HA751276) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751276) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling SLP76 with Rabbit anti-SLP76 antibody (HA751276) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLP76 antibody (HA751276) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SLP76 antibody (HA751276) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751276) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SLP76 antibody (HA751276) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751276) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
SLP76 was immunoprecipitated from 0.2 mg HL-60 cell lysate with HA751276 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751276 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HL-60 cell lysate (input) Lane 2: HA751276 IP in HL-60 cell lysate Lane 3: Rabbit IgG instead of HA751276 in HL-60 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.