DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH09-51]
cat.: HA751298
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH09-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GSDME aa 1-496. |
| Positive control: | SH-SY5Y cell lysate, SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: O60443 Human | Q9Z2D3 Mouse |
| Alternative names: | 2310037D07Rik 4932441K13Rik Deafness, autosomal dominant 5 Deafness, autosomal dominant 5 protein DFNA5 DFNA5 gene DFNA5_HUMAN Dfna5h EG14210 Fin15 ICERE 1 ICERE-1 Inversely correlated with estrogen receptor expression 1 Non-syndromic hearing impairment protein 5 Nonsyndromic hearing impairment protein |
Images
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Fig1:
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (C-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751298 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751298 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA751298 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA751298 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.