GADD45B Recombinant Rabbit Monoclonal Antibody [PSH09-52]
cat.: HA751299
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GADD45B aa 1-160. |
| Positive control: | HeLa cell lysate, Huh7 cell lysate, MCF7 cell lysate, A431 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O75293 Human | P22339 Mouse Entrez Gene: 299626 Rat |
| Alternative names: | GA45B_HUMAN GADD45 Beta Gadd45b GADD45BETA Growth arrest and DNA damage inducible 45 beta Growth arrest and DNA damage inducible beta Growth arrest and DNA damage-inducible gene GADD45, beta Growth arrest and DNA damage-inducible protein GADD45 beta Growth arrest and DNA-damage-inducible protein GADD45 beta MY118 MYD118 MYD118, mouse, homolog of Myeloid differentiation primary response gene Myeloid differentiation primary response protein MyD118 Negative growth regulatory protein MyD118 Negative growth-regulatory protein MyD118 |
Images
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Fig1:
Western blot analysis of GADD45B on different lysates with Rabbit anti-GADD45B antibody (HA751299) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (40 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751299) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.