PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-54]
cat.: HA751301
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH09-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PHOS/PDC aa 36-69/246 |
| Positive control: | Mouse eyeball tissue lysate, rat eyeball tissue lysate, mouse eyeball tissue, rat eyeball tissue. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus, Cell projection, cilium, photoreceptor outer segment, Photoreceptor inner segment, |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:2,000 1:1,000-1:8,000 1:500 1:200-1:2,000 |
| Uniprot #: | SwissProt: P20941 Human | Q9QW08 Mouse | P20942 Rat |
| Alternative names: | 33 kDa phototransducing protein MEKA protein pdc PHD PHOS_HUMAN Phosducin Protein MEKA |
Images
|
Fig1:
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/2,000 dilution. Lane 1: mouse eyeball tissue lysate (40 µg/Lane) Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: rat eyeball tissue lysate (40 µg/Lane) Lane 4: rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 33 kDa Exposure time: 3 minutes ; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751301) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751301) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunofluorescence analysis of frozen mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751301, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.