MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH09-56]
cat.: HA751303
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH09-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MMP-1 aa 1-469. |
| Positive control: | MDA-MB-231 cell lysate, U-87 MG cell lysate, HUVEC cell lysate, A375 cell lysate, human breast cancer tissue, human lung adenocarcinoma tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P03956 Human |
| Alternative names: | 27 kDa interstitial collagenase CLG CLGN collagenase, fibroblast collagenase, interstitial Fibroblast collagenase Interstitial collagenase Matrix metallopeptidase 1 (interstitial collagenase) Matrix metalloprotease 1 Matrix Metalloproteinase 1 Matrix metalloproteinase-1 MMP 1 MMP-1 MMP1 MMP1_HUMAN OTTHUMP00000045866 |
Images
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Fig1:
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (HA751303) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (negative) (20 µg/Lane) Lane 4: HUVEC cell lysate (20 µg/Lane) Lane 5: A375 cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 27 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751303) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MMP-1 antibody (HA751303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-MMP-1 antibody (HA751303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.