Bone sialoprotein 2 Recombinant Rabbit Monoclonal Antibody [PSH09-64]
cat.: HA751306
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH09-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Bone sialoprotein 2 aa 1-324/324 |
| Positive control: | Mouse articular cartilage tissue lysate, mouse embryo tissue lysate, rat embryo tissue lysate, mouse embryo tissue, rat embryo tissue. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:2,000 1:1,000 1:500 1:200 |
| Uniprot #: | SwissProt: Q61711 Mouse | P13839 Rat |
| Alternative names: | BNSP Bone sialoprotein 2 Bone sialoprotein II BSP BSP II BSPII Cell binding sialoprotein Cell-binding sialoprotein IBSP Integrin binding sialoprotein Integrin-binding sialoprotein SIAL_HUMAN SPII |
Images
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Fig1:
Western blot analysis of Bone sialoprotein 2 on different lysates with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/2,000 dilution. Lane 1: mouse articular cartilage tissue lysate Lane 2: mouse embryo tissue lysate Lane 3: rat embryo tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 3 minute ; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751306) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of frozen mouse embryonic tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751306, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751306) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Bone sialoprotein 2 antibody (HA751306) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751306) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.