MyD88 Recombinant Rabbit Monoclonal Antibody [PSH09-74]
cat.: HA751316
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH09-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MyD88 aa 101-296 / 296. |
| Positive control: | RAW264.7 cell lysate, NIH/3T3 cell lysate, A20 cell lysate, J774A.1 cell lysate, NR8383 cell lysate, C6 cell lysate, PC-12 cell lysate, A549 cell lysate, HepG2 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q99836 Human | P22366 Mouse | Q6Y1S1 Rat |
| Alternative names: | Mutant myeloid differentiation primary response 88 MYD 88 Myd88 MYD88_HUMAN MYD88D Myeloid differentiation marker 88 Myeloid differentiation primary response 88 Myeloid differentiation primary response gene (88) Myeloid differentiation primary response gene 88 Myeloid differentiation primary response gene Myeloid differentiation primary response protein MyD88 OTTHUMP00000161718 OTTHUMP00000208595 OTTHUMP00000209058 OTTHUMP00000209059 OTTHUMP00000209060 |
Images
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Fig1:
Western blot analysis of MyD88 on different lysates with Rabbit anti-MyD88 antibody (HA751316) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: A20 cell lysate Lane 4: J774A.1 cell lysate Lane 5: NR8383 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lane 8: A549 cell lysate Lane 9: HepG2 cell lysate Lane 10: COS-1 cell lysate Lane 11: Mouse liver tissue lysate Lane 12: Mouse spleen tissue lysate Lane 13: Rat liver tissue lysate Lane 14: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751316) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.