RecA Recombinant Rabbit Monoclonal Antibody [PSH09-96]
cat.: HA751327
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Escherichia coli |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH09-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within becterium RecA aa 1-353. |
| Positive control: | E.coli lysates, E.coli. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: P0A7G6 Escherichia coli |
| Alternative names: | DNA strand exchange and recombination protein with protease and nuclease activity lexB Protein recA recH Recombinase A |
Images
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Fig1:
Western blot analysis of RecA on E.coli lysates with Rabbit anti-RecA antibody (HA751327) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 42 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751327) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of E.coli labeling RecA with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.