SCO2 Recombinant Rabbit Monoclonal Antibody [PSH09-97]
cat.: HA751328
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, IP
Clonality: Monoclonal
Clone number: PSH09-97
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 30 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SCO2 aa 61-266/266
Positive control: MCF7 cell lysate, HL-60 cell lysate, SK-OV-3 cell lysate, HepG2 cell lysate, U-937 cell lysate, MCF7, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IP
1:2,000
1:50-1:200
1:100
1-2μg/sample
Uniprot #: SwissProt: O43819 Human | Q8VCL2 Mouse
Entrez Gene: 137201493 Rat
Alternative names: Cytochrome oxidase deficient homolog 2 MGC125823 MGC125825 OTTHUMP00000196774 OTTHUMP00000196775 Protein SCO2 homolog, mitochondrial SCO (cytochrome oxidase deficient, yeast) homolog 2 SCO 1L SCO 2 SCO cytochrome oxidase deficient homolog 2 (yeast) SCO cytochrome oxidase deficient homolog 2 SCO1L SCO2 SCO2_HUMAN Synthesis of cytochrome c oxidase 2
Images
HA751328_1.jpg Fig1: Western blot analysis of SCO2 on different lysates with Rabbit anti-SCO2 antibody (HA751328) at 1/2,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: SK-OV-3 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: U-937 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751328) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA751328_2.jpg Fig2: Immunocytochemistry analysis of MCF7 cells labeling SCO2 with Rabbit anti-SCO2 antibody (HA751328) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCO2 antibody (HA751328) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA751328_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SCO2 antibody (HA751328) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751328) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751328_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SCO2 antibody (HA751328) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751328_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SCO2 antibody (HA751328) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751328) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA751328_6.jpg Fig6: SCO2 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751328 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751328 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HepG2 cell lysate (input)
Lane 2: HA751328 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of HA751328 in HepG2 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 23 seconds; ECL: K1802
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.