NG2 Recombinant Rabbit Monoclonal Antibody [PSH09-98]
cat.: HA751329
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH09-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 251 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NG2 aa 1,301-2,224. |
| Positive control: | A375 cell lysate, SK-MEL-28 cell lysate, HeLa cell lysate, PANC-1 cell lysate, MDA-MB-231 cell lysate, A375. |
| Subcellular location: | Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface. |
| Recommended Dilutions:
WB IF-Cell FC |
1:10,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q6UVK1 Human |
| Alternative names: | 4732461B14Rik AN2 AN2 proteoglycan Chondroitin sulfate proteoglycan 4 (melanoma-associated) Chondroitin sulfate proteoglycan 4 Chondroitin sulfate proteoglycan NG2 Cspg4 Cspg4 chondroitin sulfate proteoglycan 4 CSPG4_HUMAN HMW-MAA HSN tumor-specific antigen Kiaa4232 MCSP MCSPG MEL-CSPG Melanoma chondroitin sulfate proteoglycan Melanoma-associated chondroitin sulfate proteoglycan MELCSPG MSK16 NG2 |
Images
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Fig1:
Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA751329) at 1/10,000 dilution. Lane 1: A375 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: HeLa cell lysate Lane 5: PANC-1 cell lysate Lane 6: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 251 kDa Observed band size: 330 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-6: 59 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751329) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A375 (positive) and SK-Br-3 (negative) labeling NG2 with Rabbit anti-NG2 antibody (HA751329) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NG2 antibody (HA751329) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SK-Br-3 (left, negative) and A375 (right, positive) cells labeling NG2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751329, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.