IRF8 Recombinant Rabbit Monoclonal Antibody [PSH10-05]
cat.: HA751335
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IRF8 aa 81-426. |
| Positive control: | Raji cell lysate, Ramos cell lysate, THP-1 cell lysate, A20 cell lysate, A20, THP-1. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:2,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q02556 Human | P23611 Mouse |
| Alternative names: | H ICSBP H-ICSBP HGNC:5358 HICSBP ICSBP 1 ICSBP ICSBP1 Interferon consensus sequence binding protein 1 Interferon consensus sequence binding protein Interferon consensus sequence-binding protein Interferon regulatory factor 8 IRF 8 IRF-8 Irf8 IRF8_HUMAN MYLS |
Images
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Fig1:
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody (HA751335) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (negative) Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 28 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751335) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody (HA751335) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A20 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751335) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A20 cells labeling IRF8 with Rabbit anti-IRF8 antibody (HA751335) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRF8 antibody (HA751335) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HCT 116 (left, negative) and THP-1 (right, positive) cells labeling IRF8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751335, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
IRF8 was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA751335 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751335 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: HA751335 IP in THP-1 cell lysate Lane 3: Rabbit IgG instead of HA751335 in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.