SMURF1 Recombinant Rabbit Monoclonal Antibody [PSH10-12]
cat.: HA751341
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMURF1 aa |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, 786-0 cell lysate, LNCaP cell lysate, U-2 OS cell lysate, A549 cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9HCE7 Human |
| Alternative names: | E3 ubiquitin-protein ligase SMURF1 hSMURF1 KIAA1625 Smad specific E3 ubiquitin ligase 1 SMAD specific E3 ubiquitin protein ligase 1 Smad ubiquitination regulatory factor 1 SMAD-specific E3 ubiquitin-protein ligase 1 SMUF1_HUMAN SMURF 1 smurf1 |
Images
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Fig1:
Western blot analysis of SMURF1 on different lysates with Rabbit anti-SMURF1 antibody (HA751341) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lane 4: 786-0 cell lysate Lane 5: LNCaP cell lysate Lane 6: U-2 OS cell lysate Lane 7: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751341) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.