Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13]
cat.: HA751342
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Integrin beta 5 aa 1-719. |
| Positive control: | A549 cell lysate, HT-29 cell lysate, NCI-H1299 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, C6 cell lysate, Rat lung tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P18084 Human Entrez Gene: 257645 Rat |
| Alternative names: | FLJ26658 Integrin beta-5 Integrin subunit beta 5 integrin, beta 5 ITB5_HUMAN Itgb5 Testis secretory sperm-binding protein Li 217p |
Images
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Fig1:
Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HT-29 cell lysate Lane 3: NCI-H1299 cell lysate Lane 4: PANC-1 cell lysate Lane 5: COS-1 cell lysate Lane 6: C6 cell lysate Lane 7: Rat lung tissue lysate Lane 8: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 1 minute 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751342) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751342) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751342) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.