Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody [PSH10-28]
cat.: HA751345
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser660 of Human HSL. |
| Positive control: | Mouse white adipose tissue lysate, Rat white adipose tissue lysate. |
| Subcellular location: | Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q05469 Human | P54310 Mouse | P15304 Rat |
| Alternative names: | Hormone sensitive lipase Hormone sensitive lipase testicular isoform Hormone-sensitive lipase HSL LHS Lipase hormone sensitive LIPE LIPS_HUMAN |
Images
|
Fig1:
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody (HA751345) at 1/2,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Rat white adipose tissue lysate Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 117 kDa Observed band size: 84 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751345) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.