HA751346

Synapsin I + II Recombinant Rabbit Monoclonal Antibody [PSH10-29]

cat.: HA751346

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IF-Cell, IHC-Fr, IHC-P, IP, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH10-29

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4).
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 74 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human Synapsin I aa 1-705.
Positive control:	Mouse brain tissue lysate, Rat brain tissue lysate, mouse primary neuronal, human brain tissue, mouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue, IMR-32 cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate.
Subcellular location:	Synapse, Golgi apparatus, Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
Recommended Dilutions: WB IF-Cell IHC-Fr IHC-P IP IF-Tissue	1:25,000 1:2,500 1:2,500 1:1,000-1:5,000 1-2μg/sample 1:200-1:1,000
Uniprot #:	SwissProt: P17600 Human \| Q92777 Human \| O88935 Mouse \| Q64332 Mouse \| P09951 Rat \| Q63537 Rat
Alternative names:	Brain protein 4.1 SYN 1 SYN 1a SYN 1b SYN I SYN1 SYN1_HUMAN SYN1a SYN1b Synapsin 1 Synapsin I Synapsin-1 Synapsin1 SynapsinI SYNI SYN 2 SYN II SYN IIa SYN IIb SYN2 SYN2_HUMAN Synapsin 2 Synapsin II Synapsin II isoform IIa Synapsin II isoform IIb Synapsin-2 Synapsin2 SynapsinII SYNII SYNIIa SYNIIb

Images

Fig1: Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat lung tissue lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 74 kDa
Observed band size: 50-74 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat lung tissue lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 74 kDa
Observed band size: 50-74 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751346, 1/2,500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
	Fig4: Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Hippocampus Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751346, 1/2,500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.
	Fig5: Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751346, 1/2,500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.

	Fig6: Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Cerebral cortex Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA751346, 1/2,500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
	Fig7: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue (negative) with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig11: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig12: Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig13: Synapsin I + II was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751346 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751346 at 1/5,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA751346 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751346 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801
	Fig14: Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution. Lane 1: IMR-32 cell lysate Lane 2: U-87 MG cell lysate Lane 3: Neuro-2a cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74/55 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.