Dopamine Transporter Recombinant Rabbit Monoclonal Antibody [PSH10-49]
cat.: HA751352
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse brain tissue, mouse striatum tissue, rat brain tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:2,000 1:500-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q61327 Mouse | P23977 Rat |
| Alternative names: | DA transporter DAT 1 DAT DAT1 Dopamine transporter 1 Dopamine transporter PKDYS SC6A3_HUMAN SLC6A3 Sodium dependent dopamine transporter Sodium-dependent dopamine transporter Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 Solute carrier family 6 (neurotransmitter transporter), member 3 Solute carrier family 6 member 3 Variable number tandem repeat (VNTR) |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Dopamine Transporter antibody (HA751352) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751352) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Dopamine Transporter antibody (HA751352) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751352) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Dopamine Transporter antibody (HA751352) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751352) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Dopamine Transporter antibody (HA751352) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751352) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Western blot analysis of Dopamine Transporter on different lysates with Rabbit anti-Dopamine Transporter antibody (HA751352) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 2: Rat brain tissue lysate (no heat) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751352) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.