Peripherin Recombinant Rabbit Monoclonal Antibody [PSH10-70]
cat.: HA751372
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH10-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human aa 1-470. |
| Positive control: | Human colon tissue, mouse colon tissue, rat colon tissue, Neuro-2a cell lysate, Mouse dorsal root ganglion tissue lysate, Neuro-2a. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, axon, Perikaryon. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Cell |
1:5,000 1:500 1:500 1:1,000 |
| Uniprot #: | SwissProt: P41219 Human | P15331 Mouse | P21807 Rat |
| Alternative names: | NEF 4 NEF4 Neurofilament 4 (57kD) Neurofilament 4 Perf PERI_HUMAN Peripherin PRPH 1 prph PRPH1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Colon Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Peripherin antibody (HA751372) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751372) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Peripherin antibody (HA751372) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751372) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Peripherin antibody (HA751372) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751372) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of Peripherin on different lysates with Rabbit anti-Peripherin antibody (HA751372) at 1/5,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: Mouse dorsal root ganglion tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 56 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751372) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig7:
Immunocytochemistry analysis of Neuro-2a cells labeling Peripherin with Rabbit anti-Peripherin antibody (HA751372) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peripherin antibody (HA751372) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.