FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-93]
cat.: HA751383
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH10-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FOXM1 aa 1-400. |
| Positive control: | Human colon tissue, human colon cancer tissue, HeLa, MEF. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q08050 Human | O08696 Mouse |
| Alternative names: | FKHL16 Forkhead box M1 Forkhead box protein M1 forkhead like 16 Forkhead-related protein FKHL16 FOX M1 Foxm1 FOXM1_HUMAN FOXM1B Hepatocyte nuclear factor 3 forkhead homolog 11 HFH-11 HFH11 HNF-3/fork-head homolog 11 HNF3 INS1 M phase phosphoprotein 2 M-phase phosphoprotein 2 MPHOSPH2 MPM-2 reactive phosphoprotein 2 MPP2 PIG29 TGT3 Transcription factor Trident Trident WIN Winged-helix factor from INS-1 cells Winged-helix factor from INS1 cells |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-FOXM1 antibody (HA751383) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751383) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FOXM1 antibody (HA751383) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751383) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling FOXM1 with Rabbit anti-FOXM1 antibody (HA751383) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXM1 antibody (HA751383) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of MEF cells labeling FOXM1 with Rabbit anti-FOXM1 antibody (HA751383) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXM1 antibody (HA751383) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.