Phospho-Rb (S608) Recombinant Rabbit Monoclonal Antibody [PSH11-04]
cat.: HA751394
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH11-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser608 of Human Rb. |
| Positive control: | K-562 cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, COS-1 cell lysate, K-562. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: P06400 Human |
| Alternative names: | Exon 17 tumor GOS561 substitution mutation causes premature stop GOS563 exon 17 substitution mutation causes premature stop OSRC Osteosarcoma p105-Rb P105RB PP105 pp110 PPP1R130 pRb Prepro retinoblastoma associated protein Protein phosphatase 1 regulatory subunit 130 Rb RB transcriptional corepressor 1 RB_HUMAN RB1 RB1 gene Retinoblastoma 1 Retinoblastoma suspectibility protein Retinoblastoma-associated protein |
Images
|
Fig1:
Western blot analysis of Phospho-Rb (S608) on different lysates with Rabbit anti-Phospho-Rb (S608) antibody (HA751394) at 1/5,000 dilution. Lane 1: K-562 cell lysate (no heat) Lane 2: SH-SY5Y cell lysate (no heat) Lane 3: MCF7 cell lysate (no heat) Lane 4: COS-1 cell lysate (no heat) Lane 5: K-562 cell lysate (no heat), the membrane treated with λpp for 1 hour Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 106 kDa Observed band size: 110 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751394) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of K-562 cells labeling Phospho-Rb (S608) with Rabbit anti-Phospho-Rb (S608) antibody (HA751394) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Rb (S608) antibody (HA751394) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187 , red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.