| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human C9orf72 aa 1-250. |
| Positive control: | SH-SY5Y cell lysate, Neuro-2a cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q96LT7 Human | Q6DFW0 Mouse | Q66HC3 Rat |
| Alternative names: | ALSFTD C9orf72 chromosome 9 open reading frame 72 CI072_HUMAN FTDALS MGC23980 Protein C9orf72 RP11-27J8.2 Uncharacterized protein C9orf72 |
|
Fig1:
Western blot analysis of C9orf72 on different lysates with Rabbit anti-C9orf72 antibody (HA751395) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Human brain tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751395) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |