Gli2 Recombinant Rabbit Monoclonal Antibody [PSH11-23]
cat.: HA751402
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH11-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 168 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Gli2 aa 571-830. |
| Positive control: | K-562 cell lysate, HT-29 cell lysate, F9 cell lysate, Mouse colon tissue lysate, Rat pancreas tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Cell projection, cilium. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P10070 Human | Q0VGT2 Mouse Entrez Gene: 304729 Rat |
| Alternative names: | CJS Gli 2 GLI family zinc finger 2 GLI Kruppel family member GLI2 GLI2 GLI2_HUMAN Glioma associated oncogene family zinc finger HPE9 Oncogene GLI2 PHS2 Tax helper protein 1 Tax helper protein 2 Tax helper protein Tax responsive element 2 holding protein Tax responsive element 25 bp sequence binding protein THP THP1 THP2 Zinc finger protein GLI2 |
Images
|
Fig1:
Western blot analysis of Gli2 on different lysates with Rabbit anti-Gli2 antibody (HA751402) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: F9 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat pancreas tissue lysate (40 µg/Lane) Lane 6: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 168 kDa Observed band size: 133 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751402) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.