Vasopressin Recombinant Rabbit Monoclonal Antibody [PSH11-64]
cat.: HA751420
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH11-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Vasopressin aa 32-124. |
| Positive control: | Mouse brain tissue, rat hypothalamus tissue, mouse hypothalamus tissue, mouse hypothalamus (paraventricular third ventricle) tissue, mouse hypothalamus (suprachiasmatic nucleus) tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-Fr IHC-P IF-Tissue |
1:500 1:50,000 1:1,000 |
| Uniprot #: | SwissProt: P01185 Human | P35455 Mouse | P01186 Rat |
| Alternative names: | ADH Antidiuretic hormone Arginine vasopressin neurophysin II ARVP AVP AVP NPII copeptin Vasopressin neurophysin II copeptin VP |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hypothalamus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. |
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Fig2:
Application: IF-tissue Species: Mouse Site: Hypothalamus Sample: Paraffin-embedded section Antibody concentration: 1,000 |
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Fig3:
Application: IF-tissue Species: Mouse Site: Hypothalamus (paraventricular third ventricle) Sample: Paraffin-embedded section Antibody concentration: 1,000 |
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Fig4:
Application: IF-tissue Species: Mouse Site: Hypothalamus (suprachiasmatic nucleus) Sample: Paraffin-embedded section Antibody concentration: 1,000 |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (HA751420) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751420) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Vasopressin antibody (HA751420) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751420) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (HA751420) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751420) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Vasopressin antibody (HA751420) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751420) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.