TFEB Recombinant Rabbit Monoclonal Antibody [PSH12-05]
cat.: HA751436
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH12-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TFEB aa 301-476. |
| Positive control: | Raji cell lysate, HeLa cell lysate, RAW264.7 cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, cytosol, Lysosome membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P19484 Human | Q9R210 Mouse Entrez Gene: 316214 Rat |
| Alternative names: | Alpha TFEB AlphaTFEB bHLHe35 Class E basic helix-loop-helix protein 35 T cell transcription factor EB TCFEB TFEB TFEB_HUMAN Transcription factor EB |
Images
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Fig1:
Western blot analysis of TFEB on different lysates with Rabbit anti-TFEB antibody (HA751436) at 1/5,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751436) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.