SLAM / CD150 Recombinant Rabbit Monoclonal Antibody [PSH12-08]
cat.: HA751437
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH12-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SLAMF1 aa 1-237. |
| Positive control: | Raji cell lysate, HUT 102 cell lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q13291 Human |
| Alternative names: | 4933415F16 AA177906 CD 150 CD150 CD150 antigen CDw150 Estm51 Ipo 3 IPO-3 Ipo3 MGC151472 MGC151476 OTTHUMP00000025670 OTTHUMP00000060252 RGD1560634 Signaling lymphocytic activation molecule Signaling lymphocytic activation molecule family member 1 Signaling lymphocytic activation molecule family member 1, isoform CRA_a Signaling lymphocytic activation molecule family member 1, isoform CRA_b Signaling lymphocytic activation molecule precursor Slaf1 SLAF1_HUMAN SLAM family, member 1 Slamf 1 SLAMF1 SLAMF1 protein |
Images
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Fig1:
Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody (HA751437) at 1/2,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: 293T cell lysate (negative) (30 µg/Lane) Lane 3: HUT 102 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (negative) (30 µg/Lane) Predicted band size: 37 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751437) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751437 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751437 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA751437 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA751437 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.