Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]
cat.: HA751447
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH12-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr18 and Ser19 of human MYL9. |
| Positive control: | PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, cell cortex. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9CQ19 Mouse | Q64122 Rat |
| Alternative names: | 20 kDa myosin light chain Human 20kDa myosin light chain (MLC2) mRNA complete cds LC20 MGC3505 MLC 2 MLC-2C MLC2 MLY 9 MRLC1 MYL9 MYL9_HUMAN Myosin light chain 9 regulatory Myosin light polypeptide 9 regulatory myosin regulatory light chain 1 Myosin regulatory light chain 2 Myosin regulatory light chain 2 smooth muscle isoform Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light polypeptide 9 Myosin RLC Myosin vascular smooth muscle light chain 2 MYRL2 OTTHUMP00000030857 smooth muscle isoform |
Images
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Fig1:
Western blot analysis of Phospho-MYL9 (T18 + S19) on different lysates with Rabbit anti-Phospho-MYL9 (T18 + S19) antibody (HA751447) at 1/5,000 dilution. Lane 1: PC-12 cell lysate Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751447) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.