Bid Recombinant Rabbit Monoclonal Antibody [PSH14-03]
cat.: HA751499
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Bid aa 51-195. |
| Positive control: | Jurkat cell lysate, HT-29 cell lysate, A549 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Cytoplasm, Mitochondrion membrane, Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P55957 Human | P70444 Mouse |
| Alternative names: | Apoptic death agonist Apoptotic death agonist BID BH3 interacting domain death agonist BH3 interacting domain death agonist p11 BH3 interacting domain death agonist p13 BH3 interacting domain death agonist p15 BH3-interacting domain death agonist p11 BID BID isoform ES(1b) BID isoform L(2) BID isoform Si6 BID_HUMAN Desmocollin type 4 FP497 Human BID coding sequence MGC15319 MGC42355 p11 BID p13 BID p15 BID p22 BID |
Images
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Fig1:
Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody (HA751499) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: A549 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751499) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Bid was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751499 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751499 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751499 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751499 in RAW264.7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.