TRAIL Recombinant Rabbit Monoclonal Antibody [PSH14-40]
cat.: HA751522
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH14-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRAIL aa 114-281. |
| Positive control: | 786-0 cell lysate, 786-0. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P50591 Human |
| Alternative names: | Apo 2 ligand APO 2L Apo-2 ligand Apo-2L APO2L CD253 CD253 antigen Chemokine tumor necrosis factor ligand superfamily member 10 Protein TRAIL TL2 TNF Related Apoptosis Inducing Ligand TNF related apoptosis inducing ligand TRAIL TNF-related apoptosis-inducing ligand TNF10_HUMAN TNFSF10 TRAIL Tumor necrosis factor (ligand) family member 10 Tumor Necrosis Factor (ligand) Superfamily Member 10 Tumor necrosis factor apoptosis inducing ligand splice variant delta Tumor necrosis factor ligand superfamily member 10 |
Images
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Fig1:
Western blot analysis of TRAIL on different lysates with Rabbit anti-TRAIL antibody (HA751522) at 1/5,000 dilution. Lane 1: 786-0 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 28/30 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751522) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa (left, negative) and 786-0 (right, positive) cells labeling TRAIL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751522, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.