Phospho-ELK1 (S383) Recombinant Rabbit Monoclonal Antibody [PSH14-65]
cat.: HA751526
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser383 of human ELK1. |
| Positive control: | RAW264.7 cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, human lung tissue untreated / treated. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P19419 Human | P41969 Mouse |
| Alternative names: | ELK 1 Elk1 ELK1 member of ETS oncogene family ELK1 protein ELK1, ETS transcription factor ELK1_HUMAN ELK2 member of ETS oncogene family ETS domain containing protein Elk 1 ETS domain containing protein Elk1 ETS domain protein Elk1 ETS domain-containing protein Elk-1 ETS like gene 1 Member of ETS oncogene family Oncogene Elk1 Tyrosine kinase (ELK1) oncogene |
Images
|
Fig1:
Western blot analysis of Phospho-ELK1 (S383) on different lysates with Rabbit anti-Phospho-ELK1 (S383) antibody (HA751526) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lane 3: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 55 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751526) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue untreated / treated with λpp with Rabbit anti-Phospho-ELK1 (S383) antibody (HA751526) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Phospho-ELK1 (S383) was immunoprecipitated from 0.2 mg RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate with HA751526 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751526 at 1/25,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (input) Lane 2: HA751526 IP in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate Lane 3: Rabbit IgG instead of HA751526 in RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 2 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.