Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66]
cat.: HA751527
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Granzyme B aa 1-247. |
| Positive control: | NK-92 cell lysate, CTLL-2 cell lysate, human NK/T-cell lymphoma tissue, human spleen tissue. |
| Subcellular location: | Secreted, Cytolytic granule. |
| Recommended Dilutions:
WB IHC-P |
1:100,000 1:500-1:5,000 |
| Uniprot #: | SwissProt: P10144 Human | P04187 Mouse |
| Alternative names: | C11 Cathepsin G like 1 Cathepsin G-like 1 CCPI CGL 1 CGL1 CSP B CSPB CTLA 1 CTLA-1 CTLA1 CTSGL1 Cytotoxic serine protease B Cytotoxic T lymphocyte associated serine esterase 1 Cytotoxic T lymphocyte proteinase 2 Cytotoxic T-lymphocyte proteinase 2 Fragmentin 2 Fragmentin-2 GRAB_HUMAN Granzyme 2 Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) Granzyme B Granzyme-2 GranzymeB GRB Gzmb Hlp Human lymphocyte protein Lymphocyte protease Protease, serine, B SECT T cell serine protease 1 3E T cell serine protease 1-3E T-cell serine protease 1-3E |
Images
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Fig1:
Western blot analysis of Granzyme B on different lysates with Rabbit anti-Granzyme B antibody (HA751527) at 1/100,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (negative) (20 µg/Lane) Lane 3: CTLL-2 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (negative) (20 µg/Lane) Predicted band size: 28 kDa Observed band size: 30 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751527) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.