RAB27B Recombinant Rabbit Monoclonal Antibody [PSH14-68]
cat.: HA751529
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RAB27B aa 1-218. |
| Positive control: | U-87 MG cell lysate, Mouse stomach tissue lysate, Rat colon tissue lysate, human colon tissue, human stomach tissue. |
| Subcellular location: | Membrane, Late endosome. |
| Recommended Dilutions:
WB IHC-P |
1:50,000 1:1,000 |
| Uniprot #: | SwissProt: O00194 Human | Q99P58 Mouse | Q99P74 Rat |
| Alternative names: | C25KG Rab27b RAB27B member RAS oncogene family RAS associated protein RAB27B Ras related protein Rab 27b Ras related protein Rab27b Ras-related protein Rab-27B RB27B_HUMAN Small GTP binding protein rab27b |
Images
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Fig1:
Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (HA751529) at 1/50,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: Mouse stomach tissue lysate (30 µg/Lane) Lane 2: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane) Lane 2: Rat colon tissue lysate (30 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751529) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RAB27B antibody (HA751529) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751529) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-RAB27B antibody (HA751529) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751529) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-RAB27B antibody (HA751529) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751529) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (HA751529) at 1/5,000 dilution. Lane 1: His-tagged RARB27A recombinant protein Lane 2: His-tagged RARB27B recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751529) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.