TDG Recombinant Rabbit Monoclonal Antibody [PSH10-11]
cat.: HA751531
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TGD aa 251-410. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, 293T cell lysate, A431 cell lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, mouse lung cancer tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:10,000 |
| Uniprot #: | SwissProt: Q13569 Human | P56581 Mouse Entrez Gene: 114521 Rat |
| Alternative names: | C JUN leucine zipper interactive protein C JUN leucine zipper interactive protein C-JUN leucine zipper interactive protein JZA-3 E130317C12Rik EC 3.2.2.29 G/T mismatch specific thymine DNA glycosylase G/T mismatch specific thymine DNA glycosylase G/T mismatch-specific thymine DNA glycosylase JZA 3 Jza1 T:G mismatch thymine glycosylase Tdg TDG_HUMAN Thymine DNA glycosylase Thymine-DNA glycosylase |
Images
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Fig1:
Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (HA751531) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Mouse spleen tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751531) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TDG on different lysates with Rabbit anti-TDG antibody (HA751531) at 1/2,000 dilution. Lane 1: B16-parental cell lysate Lane 2: B16-TDG KO cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 55 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751531) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue / TDG KO mouse lung cancer tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TDG antibody (HA751531) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751531) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.