CD62L Recombinant Rabbit Monoclonal Antibody [PSH14-92]
cat.: HA751536
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH14-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD62L aa 1-332. |
| Positive control: | Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
FC IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: P14151 Human |
| Alternative names: | A.11 AI528707 CD62 antigen ligand CD62 antigen-like family member L CD62L gp90-MEL IgA nephropathy, susceptibility to, included L Selectin L-selectin LAM-1 LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu-8 Leukocyte-endothelial cell adhesion molecule 1 Lnhr LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 LYAM1_HUMAN Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen MEL-14 Pln homing receptor PLNHR Selectin L Selectin, lymphocyte SELL TQ1 |
Images
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Fig1:
Flow cytometric analysis of 293T (left, negative) and Jurkat (right, positive) cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751536, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Immunocytochemistry analysis of Jurkat (positive) and 293T (negative) labeling CD62L with Rabbit anti-CD62L antibody (HA751536) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD62L antibody (HA751536) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.