IL-27A p28 Recombinant Rabbit Monoclonal Antibody [PSH14-93]
cat.: HA751537
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH14-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-27A p28 aa 1-234. |
| Positive control: | RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8K3I6 Mouse |
| Alternative names: | EBI3 IL 27 IL 27 p28 subunit IL 27A IL 30 IL-27 subunit alpha IL-27-A IL27 IL27-A IL27A IL27A_HUMAN IL27p28 IL30 Interleukin 27 Interleukin 27 subunit alpha Interleukin 27, 28-KD subunit Interleukin 30 Interleukin-27 subunit alpha p28 |
Images
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Fig1:
Western blot analysis of IL-27A p28 on different lysates with Rabbit anti-IL-27A p28 antibody (HA751537) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.