Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95]
cat.: HA751538
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Ly6g aa 27-105. |
| Positive control: | Mouse bone marrow, C57 mouse blood, mouse spleen tissue, mouse lung tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
FC IHC-P |
1:1,000 1:2,000-1:4,000 |
| Uniprot #: | SwissProt: P35461 Mouse |
| Alternative names: | Gr-1 Gr1 Ly-6G.1 Ly6G Lymphocyte antigen 6 complex locus G Lymphocyte antigen 6G |
Images
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Fig1:
Flow cytometric analysis of mouse bone marrow cells labeling Ly6g. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751538, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Flow cytometric analysis of C57 mouse blood cells labeling Ly6g. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751538, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA751538) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751538) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA751538) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751538) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.