CD68 Recombinant Antibody [PDM0-13] - Rabbit IgG (Chimeric) - BSA and Azide free
cat.: HA751544
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, IHC-P |
| Clone number: | PDM0-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Immunogen: | Recombinant protein within Human CD68 aa 1-354. |
| Positive control: | Human liver tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. Endosome membrane, Lysosome membrane. |
| Recommended Dilutions:
IF-Tissue IHC-P |
1:500-1:1,000 1:4,000 |
| Uniprot #: | SwissProt: P34810 Human |
| Alternative names: | CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1 |
Images
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Fig1:
Application: IF-Tissue Species: Human Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD68 antibody (HA751544) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751544) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD68 antibody (HA751544) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751544) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.