MUL1 Recombinant Rabbit Monoclonal Antibody [PSH15-17]
cat.: HA751546
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH15-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MUL1 aa 260-352. |
| Positive control: | HEK-293 cell lysate, A431 cell lysate, HeLa cell lysate, RD cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse thymus tissue lysate, Mouse kidney tissue lysate, Rat thymus tissue lysate, Rat kidney tissue lysate, human kidney tissue. |
| Subcellular location: | Mitochondrion outer membrane, Peroxisome. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q969V5 Human | Q8VCM5 Mouse Entrez Gene: 298576 Rat |
| Alternative names: | 0610009K11Rik AV000801 C1orf166 Chromosome 1 open reading frame 166 E3 SUMO-protein ligase MUL1 E3 ubiquitin ligase E3 ubiquitin protein ligase MUL1 E3 ubiquitin-protein ligase MUL1 FLJ12875 GIDE Growth inhibition and death E3 ligase MAPL Mitochondrial anchored protein ligase mitochondrial E3 ubiquitin ligase 1 mitochondrial E3 ubiquitin protein ligase 1 Mitochondrial ubiquitin ligase activator of NFKB 1 Mitochondrial-anchored protein ligase MUL1 MUL1_HUMAN MULAN Putative NF kappa B activating protein 266 Putative NF-kappa-B-activating protein 266 RGD1309944 RING finger protein 218 RNF218 RP11-401M16.2 RP23-25C1.10-002 |
Images
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Fig1:
Western blot analysis of MUL1 on different lysates with Rabbit anti-MUL1 antibody (HA751546) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: RD cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse thymus tissue lysate (low expression) (40 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Rat thymus tissue lysate (low expression) (40 µg/Lane) Lane 10: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751546) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MUL1 antibody (HA751546) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751546) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.